Insights into the regulation of Chromatin Remodelling Factors

Dr. Anton Eberharter, Adolf-Butenandt-Institut, Abteilung für Molekularbiologie, LMU München

On Wednesday November 30th, 2005 at 5:15 pm, Dr. Anton Eberharter will be our guest HPI-seminar speaker. Dr.Eberharter, currently head of a research group at the Adolf-Butenandt-Institute, Department of Molecular Biology, Munich, is an internationally very well known expert in chromatin remodelling research. A brief introduction into his research area is given below.

The packaging of eukaryotic DNA into nucleosomes and chromatin affects all nuclear processes either in a positive or negative manner. Several strategies have evolved in the cell to overcome the compaction of DNA and to regulate the fundamental chromatin-associated processes. Post-translational modifications of the histones, the ATP-dependent re-location of the nucleosomal particle along the DNA - the so-called chromatin remodelling, and the replacement of canonical histones with histone variants are among the most intensively studied mechanisms to control chromatin.

The prototype for ATP-utilizing chromatin remodelling machines is the Drosophila ATPase ISWI (Imitation SWItch). In Drosophila, ISWI resides in at least three different remodelling complexes, which show conservation from yeast to human. One of the ISWI-associated factors, dACF1, has been analyzed in vitro in great detail in the laboratory of Dr. Eberharter. dACF1 has been demonstrated to be an important modulator of the ISWI remodelling activity.

For additional information on Dr. Eberharter´s research work:

  • Hartlepp K.F., Fernandez-Tornero C., Eberharter A., Grüne T., Müller C.W. and Becker P.B. (2005) The histone fold subunits of Drosophila CHRAC facilitate nucleosome sliding through dynamic DNA interactions. Mol Cell Biol., 25, 9886-96.
  • Eberharter, A. and Becker, P.B. (2004) ATP-dependent nucleosome remodelling: factors and functions. J Cell Sci, 117, 3707-3711.
  • Eberharter, A., Vetter, I., Ferreira, R. and Becker, P.B. (2004) ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contacts. Embo J, 23, 4029-4039.
  • Eberharter, A., Längst, G. and Becker, P.B. (2004) A nucleosome sliding assay for chromatin remodeling factors. Methods Enzymol, 377, 344-353.
  • Ragvin, A., Valvatne, H., Erdal, S., Arskog, V., Tufteland, K.R., Breen, K., AM, O.Y., Eberharter, A., Gibson, T.J., Becker, P.B. and Aasland, R. (2004) Nucleosome binding by the bromodomain and PHD finger of the transcriptional cofactor p300. J Mol Biol, 337, 773-788.
  • Grüne, T., Brzeski, J., Eberharter, A., Clapier, C.R., Corona, D.F., Becker, P.B. and Muller, C.W. (2003) Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI. Mol Cell, 12, 449-460.
  • Eberharter, A. and Becker, P.B. (2002) Histone acetylation: a switch between repressive and permissive chromatin. Second in review series on chromatin dynamics. EMBO Rep, 3, 224-229.
  • Eberharter, A., Ferrari, S., Längst, G., Straub, T., Imhof, A., Varga-Weisz, P., Wilm, M. and Becker, P.B. (2001) Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling. Embo J, 20, 3781-3788.
  • Lechner, T., Carrozza, M.J., Yu, Y., Grant, P.A., Eberharter, A., Vannier, D., Brosch, G., Stillman, D.J., Shore, D. and Workman, J.L. (2000). Sds3 (suppressor of defective silencing 3) is an integral component of the yeast Sin3/Rpd3 histone deacetylase complex and is required for histone deacetylase activity. J Biol Chem, 275, 40961-40966.
  • Corona, D.F., Eberharter, A., Budde, A., Deuring, R., Ferrari, S., Varga-Weisz, P., Wilm, M., Tamkun, J. and Becker, P.B. (2000). Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC). Embo J, 19, 3049-3059.
  • Brehm, A., Längst, G., Kehle, J., Clapier, C.R., Imhof, A., Eberharter, A., Müller, J. and Becker, P.B. (2000) dMi-2 and ISWI chromatin remodelling factors have distinct nucleosome binding and mobilization properties. Embo J, 19, 4332-4341.
  • Grant, P.A., Eberharter, A., John, S., Cook, R.G., Turner, B.M. and Workman, J.L. (1999) Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem, 274, 5895-5900.
  • Ikeda, K., Steger, D.J., Eberharter, A. and Workman, J.L. (1999) Activation domain-specific and general transcription stimulation by native histone acetyltransferase complexes. Mol Cell Biol, 19, 855-863.