Staff
Prof. Dr. Uetrecht, Charlotte
Head of Associated Research Group Dynamics of Viral StructuresCurriculum vitae
Since 2021: W2 Professor in Biochemistry, Faculty V: Life Sciences, University of Siegen at the Centre for Structural Systems Biology (CSSB), Deutsches Elektronen Synchrotron (DESY) & Leibniz Institute of Virology (LIV)
2014-2021: Head of the Junior Research Group Dynamics of Viral Structures at the LIV
2011-2021: Guest scientist (employed 2013-2014) in the Sample Environment Group, European XFEL GmbH, Schenefeld, Germany
2011-2013: Postdoctorate researcher on an "EMBO longterm fellowship" with Janos Hajdu, Molecular Biophysics, Uppsala University, Sweden
2006-2010: Doctorate in the department of Albert J. R. Heck, Biomolecular Mass Spectrometry, Universiteit Utrecht, Netherlands
Publications
Dhillon A, Persson BD, Volkov AN, Sülzen H, Kádek A, Pompach P, Kereïche S, Lepšík M, Danskog K, Uetrecht C, Arnberg N, Zoll S (2024). Structural insights into the interaction between adenovirus C5 hexon and human lactoferrin. J Virol. 98/2024; PubMed
Hagan MF, Zandi R, Uetrecht C (2024). Overview of the 2023 Physical Virology Gordon Research Conference-Viruses at Multiple Levels of Complexity. Viruses. 16/2024; PubMed
Brodmerkel MN, Thiede L, De Santis E, Uetrecht C, Caleman C, Marklund EG (2024). Collision induced unfolding and molecular dynamics simulations of norovirus capsid dimers reveal strain-specific stability profiles. Phys Chem Chem Phys. 26/2024; PubMed
Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kölbel K, Uetrecht C, Thuenauer R, García-Alai MM (2024). Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast. Nat Commun. 15/2024; PubMed
Feng Y, van Bodegraven D, Kádek A, L B Munguira I, Soria-Martinez L, Nentwich S, Saha S, Chardon F, Kavan D, Uetrecht C, Schelhaas M, Roos WH (2024). Glycan-induced structural activation softens the human papillomavirus capsid for entry through reduction of intercapsomere flexibility. Nat Commun. 15/2024; PubMed
Sänger L, Williams HM, Yu D, Vogel D, Kosinski J, Rosenthal M, Uetrecht C (2023). RNA to Rule Them All: Critical Steps in Lassa Virus Ribonucleoparticle Assembly and Recruitment. J Am Chem Soc. 145/2023; PubMed
Feng Y, Pogan R, Thiede L, Müller-Guhl J, Uetrecht C, Roos WH (2023). Fucose Binding Cancels out Mechanical Differences between Distinct Human Noroviruses. Viruses. 15/2023; PubMed
Soh TK, Pfefferle S, Wurr S, von Possel R, Oestereich L, Rieger T, Uetrecht C, Rosenthal M, Bosse JB (2023). A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells. PLoS One. 18/2023; PubMed
Kierspel T, Kadek A, Barran P, Bellina B, Bijedic A, Brodmerkel MN, Commandeur J, Caleman C, Damjanović T, Dawod I, De Santis E, Lekkas A, Lorenzen K, Morillo LL, Mandl T, Marklund EG, Papanastasiou D, Ramakers LAI, Schweikhard L, Simke F, Sinelnikova A, Smyrnakis A, Timneanu N, Uetrecht C, (2023). Coherent diffractive imaging of proteins and viral capsids: simulating MS SPIDOC. Anal Bioanal Chem. 415/2023; PubMed
Brodmerkel MN, De Santis E, Uetrecht C, Caleman C, Marklund EG (2022). Stability and conformational memory of electrosprayed and rehydrated bacteriophage MS2 virus coat proteins. Curr Res Struct Biol. 4/2022; PubMed
Shukla S, Komarek J, Novakova Z, Nedvedova J, Ustinova K, Vankova P, Kadek A, Uetrecht C, Mertens H, Barinka C (2022). In-solution structure and oligomerization of human histone deacetylase 6 - an integrative approach. FEBS J. 290/2023; PubMed
Creutznacher R, Maass T, Veselkova B, Ssebyatika G, Krey T, Empting M, Tautz N, Frank M, Kölbel K, Uetrecht C, Peters T (2022). NMR Experiments Provide Insights into Ligand-Binding to the SARS-CoV-2 Spike Protein Receptor-Binding Domain. J Am Chem Soc. 144/2022; PubMed
Creutznacher R, Maass T, Dülfer J, Feldmann C, Hartmann V, Lane MS, Knickmann J, Westermann LT, Thiede L, Smith TJ, Uetrecht C, Mallagaray A, Waudby CA, Taube S, Peters T (2022). Distinct dissociation rates of murine and human norovirus P-domain dimers suggest a role of dimer stability in virus-host interactions. Commun Biol. 5/2022; PubMed
Kopicki JD, Saikia A, Niebling S, Günther C, Anjanappa R, Garcia-Alai M, Springer S, Uetrecht C (2022). Opening opportunities for Kd determination and screening of MHC peptide complexes. Commun Biol. 5/2022; PubMed
Dirscherl C, Löchte S, Hein Z, Kopicki JD, Harders AR, Linden N, Karner A, Preiner J, Weghuber J, Garcia-Alai M, Uetrecht C, Zacharias M, Piehler J, Lanzerstorfer P, Springer S (2022). Dissociation of β2m from MHC class I triggers formation of noncovalent transient heavy chain dimers. J Cell Sci. 135/2022; PubMed
Maass T, Westermann LT, Creutznacher R, Mallagaray A, Dülfer J, Uetrecht C, Peters T (2022). Assignment of Ala, Ile, LeuproS, Met, and ValproS methyl groups of the protruding domain of murine norovirus capsid protein VP1 using methyl-methyl NOEs, site directed mutagenesis, and pseudocontact shifts. Biomol NMR Assign. 16/2022; PubMed
Peters T, Creutznacher R, Maass T, Mallagaray A, Ogrissek P, Taube S, Thiede L, Uetrecht C (2022). Norovirus-glycan interactions - how strong are they really? Biochem Soc Trans. 50/2022; PubMed
Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M (2021). Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines. EMBO J. 41/2022; PubMed
Kadek A, Lorenzen K, Uetrecht C, (2021). In a flash of light: X-ray free electron lasers meet native mass spectrometry. Drug Discov Today Technol. 39/2021; PubMed
Yan H, Lockhauserbäumer J, Szekeres GP, Mallagaray A, Creutznacher R, Taube S, Peters T, Pagel K, Uetrecht C (2021). Protein Secondary Structure Affects Glycan Clustering in Native Mass Spectrometry. Life (Basel). 11/2021; PubMed
Simanjuntak Y, Schamoni-Kast K, Grün A, Uetrecht C, Scaturro P (2021). Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology. Viruses. 13/2021; PubMed
Dülfer J, Yan H, Brodmerkel MN, Creutznacher R, Mallagaray A, Peters T, Caleman C, Marklund EG, Uetrecht C (2021). Glycan-Induced Protein Dynamics in Human Norovirus P Dimers Depend on Virus Strain and Deamidation Status. Molecules. 26/2021; PubMed
Han H, Round E, Schubert R, Gül Y, Makroczyová J, Meza D, Heuser P, Aepfelbacher M, Barák I, Betzel C, Fromme P, Kursula I, Nissen P, Tereschenko E, Schulz J, Uetrecht C, Ulicný J, Wilmanns M, Hajdu J, Lamzin VS, Lorenzen K (2021). The XBI BioLab for life science experiments at the European XFEL. J Appl Crystallogr. 54/2021; PubMed
Günther S, Reinke PYA, Fernández-García Y, Lieske J, Lane TJ, Ginn HM, Koua FHM, Ehrt C, Ewert W, Oberthuer D, Yefanov O, Meier S, Lorenzen K, Krichel B, Kopicki JD, Gelisio L, Brehm W, Dunkel I, Seychell B, Gieseler H, Norton-Baker B, Escudero-Pérez B, Domaracky M, Saouane S, Tolstikova A, White TA, Hänle A, Groessler M, Fleckenstein H, Trost F, Galchenkova M, Gevorkov Y, Li C, Awel S, Peck A, Barthelmess M, Schlünzen F, Lourdu Xavier P, Werner N, Andaleeb H, Ullah N, Falke S, Srinivasan V, França BA, Schwinzer M, Brognaro H, Rogers C, Melo D, Zaitseva-Kinneberg JI, Knoska J, Peña-Murillo GE, Mashhour AR, Hennicke V, Fischer P, Hakanpää J, Meyer J, Gribbon P, Ellinger B, Kuzikov M, Wolf M, Beccari AR, Bourenkov G, von Stetten D, Pompidor G, Bento I, Panneerselvam S, Karpics I, Schneider TR, Garcia-Alai MM, Niebling S, Günther C, Schmidt C, Schubert R, Han H, Boger J, Monteiro DCF, Zhang L, Sun X, Pletzer-Zelgert J, Wollenhaupt J, Feiler CG, Weiss MS, Schulz EC, Mehrabi P, Karničar K, Usenik A, Loboda J, Tidow H, Chari A, Hilgenfeld R, Uetrecht C, Cox R, Zaliani A, Beck T, Rarey M, Günther S, Turk D, Hinrichs W, Chapman HN, Pearson AR, Betzel C, Meents A (2021). X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease. Science. 372/2021; PubMed
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C (2021). Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes. Sci Adv. 7/2021; PubMed
Pogan R, Weiss VU, Bond K, Dülfer J, Krisp C, Lyktey N, Müller-Guhl J, Zoratto S, Allmaier G, Jarrold MF, Muñoz-Fontela C, Schlüter H, Uetrecht C (2020). N-terminal VP1 Truncations Favor T = 1 Norovirus-Like Particles. Vaccines (Basel). 9/2020; PubMed
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C (2020). Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes. bioRxiv. 2020 Oct 07; PubMed
Schulz EC, Henderson SR, Illarionov B, Crosskey T, Southall SM, Krichel B, Uetrecht C, Fischer M, Wilmanns M (2020). The crystal structure of mycobacterial epoxide hydrolase A. Sci Rep. 10/2020; PubMed
Horstmann JA, Lunelli M, Cazzola H, Heidemann J, Kühne C, Steffen P, Szefs S, Rossi C, Lokareddy RK, Wang C, Lemaire L, Hughes KT, Uetrecht C, Schlüter H, Grassl GA, Stradal TEB, Rossez Y, Kolbe M, Erhardt M (2020). Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion. Nat Commun. 11/2020; PubMed
Anjanappa R, Garcia-Alai M, Kopicki JD, Lockhauserbäumer J, Aboelmagd M, Hinrichs J, Nemtanu IM, Uetrecht C, Zacharias M, Springer S, Meijers R (2020). Structures of peptide-free and partially loaded MHC class I molecules reveal mechanisms of peptide selection. Nat Commun. 11/2020; PubMed
Krichel B, Falke S, Hilgenfeld R, Redecke L, Uetrecht C (2020). Processing of the SARS-CoV pp1a/ab nsp7-10 region. Biochem J. 477/2020; PubMed
Heidemann J, Kölbel K, Konijnenberg A, Van Dyck J, Garcia-Alai M, Meijers R, Sobott F, Uetrecht C (2019). Further insights from structural mass spectrometry into endocytosis adaptor protein assemblies. Int J Mass Spectrom. 447/2020; PubMed
Dülfer J, Kadek A, Kopicki JD, Krichel B, Uetrecht C (2019). Structural mass spectrometry goes viral. Adv Virus Res. 105/2019; PubMed
Bernal I, Römermann J, Flacht L, Lunelli M, Uetrecht C, Kolbe M (2019). Structural analysis of ligand-bound states of the Salmonella type III secretion system ATPase InvC. Protein Sci. 28/2019; PubMed
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M (2019). Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol. 431/2019; PubMed
Weiss VU, Pogan R, Zoratto S, Bond KM, Boulanger P, Jarrold MF, Lyktey N, Pahl D, Puffler N, Schelhaas M, Selivanovitch E, Uetrecht C, Allmaier G (2019). Virus-like particle size and molecular weight/mass determination applying gas-phase electrophoresis (native nES GEMMA). Anal Bioanal Chem. 411/2019; PubMed
Uetrecht C, Lorenzen K, Kitel M, Heidemann J, Robinson Spencer JH, Schlüter H, Schulz J (2019). Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging. J Synchrotron Radiat. 26/2019; PubMed
Mallagaray A, Creutznacher R, Dülfer J, Mayer PHO, Grimm LL, Orduña JM, Trabjerg E, Stehle T, Rand KD, Blaum BS, Uetrecht C, Peters T (2019). A post-translational modification of human Norovirus capsid protein attenuates glycan binding. Nat Commun. 10/2019; PubMed
Zaitsev-Doyle JJ, Puchert A, Pfeifer Y, Yan H, Yorke BA, Müller-Werkmeister HM, Uetrecht C, Rehbein J, Huse N, Pearson AR, Sans M (2019). Synthesis and characterisation of α-carboxynitrobenzyl photocaged l-aspartates for applications in time-resolved structural biology. RSC Adv. 9/2019; PubMed
Kesgin-Schaefer S, Heidemann J, Puchert A, Koelbel K, Yorke BA, Huse N, Pearson AR, Uetrecht C, Tidow H (2019). Crystal structure of a domain-swapped photoactivatable sfGFP variant provides evidence for GFP folding pathway. FEBS J. 286/2019; PubMed
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H (2018). Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol. 1/2018; PubMed
Holm T, Kopicki JD, Busch C, Olschewski S, Rosenthal M, Uetrecht C, Günther S, Reindl S (2018). Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses. J Biol Chem. 293/2018; PubMed
Bücher KS, Yan H, Creutznacher R, Ruoff K, Mallagaray A, Grafmüller A, Dirks JS, Kilic T, Weickert S, Rubailo A, Drescher M, Schmidt S, Hansman G, Peters T, Uetrecht C, Hartmann L (2018). Fucose-Functionalized Precision Glycomacromolecules Targeting Human Norovirus Capsid Protein. Biomacromolecules. 19/2018; PubMed
Pogan R, Dülfer J, Uetrecht C (2018). Norovirus assembly and stability. Curr Opin Virol. 31/2018; PubMed
Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R (2018). Epsin and Sla2 form assemblies through phospholipid interfaces. Nat Commun. 9/2018; PubMed
Wegener H, Mallagaray Á, Schöne T, Peters T, Lockhauserbäumer J, Yan H, Uetrecht C, Hansman GS, Taube S (2017). Human norovirus GII.4(MI001) P dimer binds fucosylated and sialylated carbohydrates. Glycobiology. 27/2017; PubMed
Shin HC, Deterra D, Park J, Kim H, Nishikiori M, Uetrecht C, Ahlquist PG, Arbulu M, Blick RH (2017). Ultra-high mass multimer analysis of protein-1a capping domains by a silicon nanomembrane detector. J Proteomics. 175/2018; PubMed
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U (2016). Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol. 13/2016; PubMed
Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R (2015). Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem. 291/2016; PubMed
Mallagaray A, Lockhauserbäumer J, Hansman G, Uetrecht C, Peters T (2015). Attachment of norovirus to histo blood group antigens: a cooperative multistep process. Angew Chem Int Ed Engl. 54/2015; PubMed