© 2013 Leibniz-Institut für Virologie
Methods
- tissue culture techniques
- transfection/infection of mamalian cells
- transformation reporter gene assays in primary mamalian cells
- RNA interference (RNAi) technology
- fluorescence microscopy
- multicolor flow cytomerty
- protein/protein nteractions
- DNA/protein interactions (chromatin immunoprecipitation, ChIP)
- 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
- expression and purification of recombinant proteins
- generation and characterization of novel antibodies
- isolation, identification, high-titer production of viruses
- phentotypic characterization
- generation of recombinant adenoviral vectors/oncolytic viruses
- quantitative real time PCR
- FISH (Fluoreszenz in situ hybridisation)
- generation and transduction of recombinant lentiviral particles
- humanized mouse models of infection/bone marrow chimeric mice
Recent Publications
Dobner T, Bertzbach LD. (2023). Updates and New Perspectives on Adenoviral Gene Therapy and Vaccine Vectors. Viruses. 2023 Feb 13;15(2):514.
Ip WH, Bertzbach LD, Speiseder T, Dobner T. (2023). The adenoviral E4orf3/4 is a regulatory polypeptide with cell transforming properties in vitro. Tumour Virus Res.2023 Jan 25;15:200254.
Alemán MV, Bertzbach LD, Speiseder T, Ip WH, González RA, Dobner T (2022). Global Transcriptome Analyses of Cellular and Viral mRNAs during HAdV-C5 Infection Highlight New Aspects of Viral mRNA Biogenesis and Cytoplasmic Viral mRNA Accumulations Viruses. 2022; 14(11):2428.