• tissue culture techniques
    • transfection/infection of mamalian cells
    • transformation reporter gene assays in primary mamalian cells
    • RNA interference (RNAi) technology
    • fluorescence microscopy
    • multicolor flow cytomerty
    • protein/protein nteractions
    • DNA/protein interactions (chromatin immunoprecipitation, ChIP)
    • 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
    • expression and purification of recombinant proteins
    • generation and characterization of novel antibodies
    • isolation, identification, high-titer production of viruses
    • phentotypic characterization
    • generation of recombinant  adenoviral vectors/oncolytic viruses
    • quantitative real time PCR
    • FISH (Fluoreszenz in situ hybridisation)
    • generation and transduction of recombinant lentiviral particles
    • humanized mouse models of infection/bone marrow chimeric mice

        Recent Publications

        Dobner T, Bertzbach LD. (2023). Updates and New Perspectives on Adenoviral Gene Therapy and Vaccine Vectors. Viruses. 2023 Feb 13;15(2):514.

        Ip WH, Bertzbach LD, Speiseder T, Dobner T. (2023). The adenoviral E4orf3/4 is a regulatory polypeptide with cell transforming properties in vitro. Tumour Virus Res.2023 Jan 25;15:200254.

        Alemán MV, Bertzbach LD, Speiseder T, Ip WH, González RA, Dobner T (2022). Global Transcriptome Analyses of Cellular and Viral mRNAs during HAdV-C5 Infection Highlight New Aspects of Viral mRNA Biogenesis and Cytoplasmic Viral mRNA Accumulations Viruses. 2022; 14(11):2428.