Methods
- Mammalian cell culture (primary cells and cell lines)
- Tissue processing (liver, gut, spleen)
- Tissue-culture techniques under S2 and S3** conditions
- Single-cell cloning
- HIV-1 in vitro infection models
- HIV-1 latency models
- Assessment of NK cell function
- Jurkat-Reporter assay (KIR/HLA interactions)
- HLA stabilization assay
- Generation of HLA Class I tetramers
- Multiparameter flow cytometry (20 parameters)
- Fluorescent-activated cell sorting (S3**, 4-way, 96-well, single cell)
- RNA Flow cytometry (Prime Flow)
- Chipcytometry
- Seahorse Assay (Metabolism)
- Multiplex immunoassays for Luminex
- Molecular-biological and biochemical methods (DNA/RNA/protein)
- RNA interference (siRNA)
- Genome-wide CRISPR knock-out screen (GeCKO)/CRISPR/Cas9
- Single-cell genomics/ single-cell transcriptome analysis (Fluidigm)
- Organoid techniques including parenchym-immune cell co-cultures in organoid systems
- Viral infection of primary cells in organoids
Publications (Selection)
Beisel C, Jordan-Paiz A, Köllmann S, Ahrenstorf AE, Padoan B, Barkhausen T, Addo MM, Altfeld M. (2023). Sex differences in the percentage of IRF5 positive B cells are associated with higher production of TNF-α in women in response to TLR9 in humans. Biol Sex Differ. 2023 Feb 22;14(1):11.
Kroll MK, Schloer S, Candan P, Korthals N, Wenzel C, Ihle H, Gilhaus K, Liedtke KR, Schöfbänker M, Surmann B, Schröter R, Neugebauer U, Mall G, Oswald S, Ludwig S, Rescher U, Vollenbröker B, Ciarimboli G. (2023). Importance of ACE2 for SARS-CoV-2 Infection of Kidney Cells. Biomolecules. 2023 Mar 3;13(3):472.
Ufer F, Ziegler SM, Altfeld M, Friese MA. (2023). Case report: JAK inhibition as promising treatment option of fatal RVCLS due to TREX1 mutation (pVAL235Glyfs*6). Front Neurol. 2023 Feb 21;14:1118369.