Methods
- Mammalian cell culture (primary cells and cell lines)
- Tissue processing (liver, gut, spleen)
- Tissue-culture techniques under S2 and S3** conditions
- Single-cell cloning
- HIV-1 in vitro infection models
- HIV-1 latency models
- Assessment of NK cell function
- Jurkat-Reporter assay (KIR/HLA interactions)
- HLA stabilization assay
- Generation of HLA Class I tetramers
- Multiparameter flow cytometry (20 parameters)
- Fluorescent-activated cell sorting (S3**, 4-way, 96-well, single cell)
- RNA Flow cytometry (Prime Flow)
- Chipcytometry
- Seahorse Assay (Metabolism)
- Multiplex immunoassays for Luminex
- Molecular-biological and biochemical methods (DNA/RNA/protein)
- RNA interference (siRNA)
- Genome-wide CRISPR knock-out screen (GeCKO)/CRISPR/Cas9
- Single-cell genomics/ single-cell transcriptome analysis (Fluidigm)
- Organoid techniques including parenchym-immune cell co-cultures in organoid systems
- Viral infection of primary cells in organoids
Publications (Selection)
Akiso M, Ameka M, Naidoo K, Langat R, Kombo J, Sikuku D, Ndung'u T, Altfeld M, Anzala O, Mureithi M. (2023). Metabolic and mitochondrial dysregulation in CD4+ T cells from HIV-positive women on combination anti-retroviral therapy. PLoS One. 2023 Oct 10;18(10):e0286436.
Baumdick ME, Niehrs A, Degenhardt F, Schwerk M, Hinrichs O, Jordan-Paiz A, Padoan B, Wegner LHM, Schloer S, (..), Altfeld M, Bunders MJ. (2023). HLA-DP on Epithelial Cells enables Tissue Damage by NKp44+ NK Cells in Ulcerative Colitis. Gastroenterology. 2023 Jul 14:S0016-5085(23)04772-8.
Beisel C, Jordan-Paiz A, Köllmann S, Ahrenstorf AE, Padoan B, Barkhausen T, Addo MM, Altfeld M. (2023). Sex differences in the percentage of IRF5 positive B cells are associated with higher production of TNF-α in women in response to TLR9 in humans. Biol Sex Differ. 2023 Feb 22;14(1):11.