Methods
- tissue culture techniques
- transfection/infection of mamalian cells
- transformation reporter gene assays in primary mamalian cells
- RNA interference (RNAi) technology
- fluorescence microscopy
- multicolor flow cytomerty
- protein/protein nteractions
- DNA/protein interactions (chromatin immunoprecipitation, ChIP)
- 2D-gelelektrophoresis/ MALDI-ToF mass spectroscopy
- expression and purification of recombinant proteins
- generation and characterization of novel antibodies
- isolation, identification, high-titer production of viruses
- phentotypic characterization
- generation of recombinant adenoviral vectors/oncolytic viruses
- quantitative real time PCR
- FISH (Fluoreszenz in situ hybridisation)
- generation and transduction of recombinant lentiviral particles
- humanized mouse models of infection/bone marrow chimeric mice
Contact
Head of Research Department
Phone: +49 (0)40 48051-300/301
Email: thomas.dobner@leibniz-liv.de
