Methods
- Mammalian cell culture (primary cells and cell lines)
- Tissue processing (liver, gut, spleen)
- Tissue-culture techniques under S2 and S3** conditions
- Single-cell cloning
- HIV-1 in vitro infection models
- HIV-1 latency models
- Assessment of NK cell function
- Jurkat-Reporter assay (KIR/HLA interactions)
- HLA stabilization assay
- Generation of HLA Class I tetramers
- Multiparameter flow cytometry (20 parameters)
- Fluorescent-activated cell sorting (S3**, 4-way, 96-well, single cell)
- RNA Flow cytometry (Prime Flow)
- Chipcytometry
- Seahorse Assay (Metabolism)
- Multiplex immunoassays for Luminex
- Molecular-biological and biochemical methods (DNA/RNA/protein)
- RNA interference (siRNA)
- Genome-wide CRISPR knock-out screen (GeCKO)/CRISPR/Cas9
- Single-cell genomics/ single-cell transcriptome analysis (Fluidigm)
- Organoid techniques including parenchym-immune cell co-cultures in organoid systems
- Viral infection of primary cells in organoids
Contact
Head of Research Department
Phone: +49 (0)40 48051-221
Team Assistance
Phone: +49 (0)40 48051-229
