Mitarbeiterinnen und Mitarbeiter
Prof. Dr. Grünewald, Kay
Abteilungsleiter Strukturelle Zellbiologie der VirenCurriculum vitae
2024-2025: Deputy Scientific Director; Centre for Structural Systems Biology (CSSB), Hamburg, Germany
2022-2023: Scientific Director; Centre for Structural Systems Biology (CSSB), Hamburg, Germany
2020-2021: Deputy Scientific Director; Centre for Structural Systems Biology (CSSB), Hamburg, Germany
2017-2021: Visiting Professor of Structural Cell Biology; University of Oxford, United Kingdom
Since 2014: Faculty Member, Director of multi-user Electron Cryo-Microscopy (Cryo-EM) Facility, and Co-Director of Advanced Light and Fluorescence Microscopy (ALFM) Facility; Centre for Structural Systems Biology (CSSB), Hamburg, Germany
2013-2018: Associated Scientist; Diamond Light Source, Harwell, United Kingdom
2013-2017: Professor of Structural Cell Biology; University of Oxford, United Kingdom
2009-2017: Senior Group Leader and Head of Oxford Particle Imaging Centre; University of Oxford, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom
2004-2009: Junior Research Group Leader; Emmy Noether Group of German Research Foundation (DFG), Max Planck Institute of Biochemistry, Martinsried, Germany
2004: Scientist; Max Planck Institute of Biochemistry, Martinsried, Germany
2002-2003: Postdoctoral Fellow; National Institutes of Health, Bethesda, USA with Dr. Alasdair C. Steven (Laboratory Structural Biology Research)
2001: Postdoctoral Fellow; Friedrich Schiller University Jena, and Max Planck Institute of Biochemistry, Martinsried, Germany with Prof. Wolfgang Baumeister
1997-2000: Doctorate in Biology (Dr. rer. nat.) with Prof. Wolfram Braune, Friedrich Schiller University Jena, Germany; 1997/1998 research stays with Prof. Joseph Hirschberg, Hebrew University of Jerusalem, Israel
1991-1996: Studies of Biology (Diploma), Friedrich Schiller University Jena, Germany
Publikationen
Manalastas-Cantos K, Adoni KR, Pfeifer M, Märtens B, Grünewald K, Thalassinos K, Topf M (2024). Modeling Flexible Protein Structure With AlphaFold2 and Crosslinking Mass Spectrometry. Mol Cell Proteomics. 23/2024; PubMed
Pražák V, Mironova Y, Vasishtan D, Hagen C, Laugks U, Jensen Y, Sanders S, Heumann JM, Bosse JB, Klupp BG, Mettenleiter TC, Grange M, Grünewald K (2024). Molecular plasticity of herpesvirus nuclear egress analysed in situ. Nat Microbiol. 9/2024; PubMed
Gustavsson E, Grünewald K, Elias P, Hällberg BM (2024). Dynamics of the Herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-reading revealed by Cryo-EM. Nucleic Acids Res. 52/2024; PubMed
Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grünewald K, Quemin ERJ, Rosenthal M (2024). Structural snapshots of phenuivirus cap-snatching and transcription. Nucleic Acids Res. 52/2024; PubMed
Liu J, Corroyer-Dulmont S, Pražák V, Khusainov I, Bahrami K, Welsch S, Vasishtan D, Obarska-Kosińska A, Thorkelsson SR, Grünewald K, Quemin ERJ, Turoňová B, Locker JK (2024). The palisade layer of the poxvirus core is composed of flexible A10 trimers. Nat Struct Mol Biol. 31/2024; PubMed
Ott F, Jönsson MR, Grünewald K, Hellert J (2024). Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography. Methods Mol Biol. 2824/2024; PubMed
Hofstadter WA, Cook KC, Tsopurashvili E, Gebauer R, Pražák V, Machala EA, Park JW, Grünewald K, Quemin ERJ, Cristea IM (2024). Infection-induced peripheral mitochondria fission drives ER encapsulations and inter-mitochondria contacts that rescue bioenergetics. Nat Commun. 15/2024; PubMed
Zhou M, Vollmer B, Machala E, Chen M, Grünewald K, Arvin AM, Chiu W, Oliver SL (2023). Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nat Commun. 14/2023; PubMed
Meier K, Thorkelsson SR, Durieux Trouilleton Q, Vogel D, Yu D, Kosinski J, Cusack S, Malet H, Grünewald K, Quemin ERJ, Rosenthal M (2023). Structural and functional characterization of the Sin Nombre virus L protein. PLoS Pathog. 19/2023; PubMed
Ferreira JL, Pražák V, Vasishtan D, Siggel M, Hentzschel F, Binder AM, Pietsch E, Kosinski J, Frischknecht F, Gilberger TW, Grünewald K (2023). Variable microtubule architecture in the malaria parasite. Nat Commun. 14/2023; PubMed
Williams HM, Thorkelsson SR, Vogel D, Milewski M, Busch C, Cusack S, Grünewald K, Quemin ERJ, Rosenthal M (2023). Structural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein. Nucleic Acids Res. 51/2023; PubMed
Franken LE, Rosch R, Laugks U, Grünewald K (2022). Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells. STAR Protoc. 3/2022; PubMed
Bücker R, Seuring C, Cazey C, Veith K, García-Alai M, Grünewald K, Landau M (2022). The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils. Nat Commun. 13/2022; PubMed
Caragliano E, Bonazza S, Frascaroli G, Tang J, Soh TK, Grünewald K, Bosse JB, Brune W (2022). Human cytomegalovirus forms phase-separated compartments at viral genomes to facilitate viral replication. Cell Rep. 38/2022; PubMed
Flomm FJ, Soh TK, Schneider C, Wedemann L, Britt HM, Thalassinos K, Pfitzner S, Reimer R, Grünewald K, Bosse JB (2022). Intermittent bulk release of human cytomegalovirus. PLoS Pathog. 18/2022; PubMed
Villalta A, Schmitt A, Estrozi LF, Quemin ERJ, Alempic JM, Lartigue A, Pražák V, Belmudes L, Vasishtan D, Colmant AMG, Honoré FA, Couté Y, Grünewald K, Abergel C (2022). The giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield. Elife. 11/2022; PubMed
Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M (2021). Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines. EMBO J. 41/2022; PubMed
Pfitzner S, Bosse JB, Hofmann-Sieber H, Flomm F, Reimer R, Dobner T, Grünewald K, Franken LE (2021). Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. Int J Mol Sci. 22/2021; PubMed
Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Günther S, Grünewald K, Rosenthal M, Cusack S (2021). Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity. Nat Commun. 12/2021; PubMed
Vallbracht M, Lötzsch H, Klupp BG, Fuchs W, Vollmer B, Grünewald K, Backovic M, Rey FA, Mettenleiter TC (2021). In Vitro Viral Evolution Identifies a Critical Residue in the Alphaherpesvirus Fusion Glycoprotein B Ectodomain That Controls gH/gL-Independent Entry. mBio. 12/2021; PubMed
Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA (2021). DNA origami signposts for identifying proteins on cell membranes by electron cryotomography. Cell. 184/2021; PubMed
Pražák V, Grünewald K, Kaufmann R (2020). Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI. Methods Cell Biol. 162/2021; PubMed
Wolff G, Limpens RWAL, Zevenhoven-Dobbe JC, Laugks U, Zheng S, de Jong AWM, Koning RI, Agard DA, Grünewald K, Koster AJ, Snijder EJ, Bárcena M (2020). A molecular pore spans the double membrane of the coronavirus replication organelle. Science. 369/2020; PubMed
Quemin ERJ, Machala EA, Vollmer B, Pražák V, Vasishtan D, Rosch R, Grange M, Franken LE, Baker LA, Grünewald K (2020). Cellular Electron Cryo-Tomography to Study Virus-Host Interactions. Annu Rev Virol. 7/2020; PubMed
Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020). Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'. PLoS Pathog. 16/2020; PubMed
Vogel D, Thorkelsson SR, Quemin ERJ, Meier K, Kouba T, Gogrefe N, Busch C, Reindl S, Günther S, Cusack S, Grünewald K, Rosenthal M (2020). Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein. Nucleic Acids Res. 48/2020; PubMed
Chorev DS, Tang H, Rouse SL, Bolla JR, von Kügelgen A, Baker LA, Wu D, Gault J, Grünewald K, Bharat TAM, Matthews SJ, Robinson CV (2020). The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes. Nat Protoc. 15/2020; PubMed
Franken LE, Grünewald K, Boekema EJ, Stuart MCA (2020). A Technical Introduction to Transmission Electron Microscopy for Soft-Matter: Imaging, Possibilities, Choices, and Technical Developments. Small. 16/2020; PubMed
Vollmer B, Grünewald K (2020). Herpesvirus membrane fusion - a team effort. Curr Opin Struct Biol. 62/2020; PubMed
Vollmer B, Pražák V, Vasishtan D, Jefferys EE, Hernandez-Duran A, Vallbracht M, Klupp BG, Mettenleiter TC, Backovic M, Rey FA, Topf M, Grünewald K (2020). The prefusion structure of herpes simplex virus glycoprotein B. Sci Adv. 6/2020; PubMed
Hernández Durán A, Grünewald K, Topf M (2019). Conserved Central Intraviral Protein Interactome of the Herpesviridae Family. mSystems. 4/2019; PubMed
Hernández Durán A, Greco TM, Vollmer B, Cristea IM, Grünewald K, Topf M (2019). Protein interactions and consensus clustering analysis uncover insights into herpesvirus virion structure and function relationships. PLoS Biol. 17/2019; PubMed
Moser F, Pražák V, Mordhorst V, Andrade DM, Baker LA, Hagen C, Grünewald K, Kaufmann R (2019). Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy. Proc Natl Acad Sci U S A. 116/2019; PubMed
Grünewald K, Krey T (2018). Editorial Overview. Curr Opin Virol. 31/2018; PubMed
Chorev DS, Baker LA, Wu D, Beilsten-Edmands V, Rouse SL, Zeev-Ben-Mordehai T, Jiko C, Samsudin F, Gerle C, Khalid S, Stewart AG, Matthews SJ, Grünewald K, Robinson CV (2018). Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry. Science. 362/2018; PubMed
Ando T, Bhamidimarri SP, Brending N, Colin-York H, Collinson L, De Jonge N, de Pablo PJ, Debroye E, Eggeling C, Franck C, Fritzsche M, Gerritsen H, Giepmans BNG, Grunewald K, Hofkens J, Hoogenboom JP, Janssen KPF, Kaufman R, Klumpermann J, Kurniawan N, Kusch J, Liv N, Parekh V, Peckys DB, Rehfeldt F, Reutens DC, Roeffaers MBJ, Salditt T, Schaap IAT, Schwarz US, Verkade P, Vogel MW, Wagner R, Winterhalter M, Yuan H, Zifarelli G (2018). The 2018 correlative microscopy techniques roadmap. J Phys D Appl Phys. 51/2018; PubMed
Montespan C, Marvin SA, Austin S, Burrage AM, Roger B, Rayne F, Faure M, Campell EM, Schneider C, Reimer R, Grünewald K, Wiethoff CM, Wodrich H (2017). Multi-layered control of Galectin-8 mediated autophagy during adenovirus cell entry through a conserved PPxY motif in the viral capsid. PLoS Pathog. 13/2017; PubMed
