Mitarbeiterinnen und Mitarbeiter

Prof. Dr. Grünewald, Kay

Abteilungsleiter Strukturelle Zellbiologie der Viren

+49 (0)40 8998-87700

Lebenslauf

Since 2017: Professor (W3) for Structural Virology; Institute of Biochemistry and Molecular Biology, University of Hamburg, Hamburg, Germany

Since 2017: Head of Research Department ‘Structural Cell Biology of Viruses’, Leibniz Institute of Virology, Hamburg, Germany

20172024–2025: Stellvertretender Wissenschaftlicher Direktor am Centre for Structural Systems Biology (CSSB), Hamburg, Deutschland

2022–2023: Wissenschaftlicher Direktor am Centre for Structural Systems Biology (CSSB), Hamburg, Deutschland

2020–2021: Stellvertretender Wissenschaftlicher Direktor am Centre for Structural Systems Biology (CSSB), Hamburg, Deutschland

2017–2021: Visiting Professor für Strukturelle Zellbiologie, University of Oxford, Oxford, Vereinigtes Königreich

Seit 2014: Fakultätsmitglied, Direktor der Multi-User Electron Cryo-Microscopy (Cryo-EM) Facility sowie Co-Direktor der Advanced Light and Fluorescence Microscopy (ALFM) Facility am Centre for Structural Systems Biology (CSSB), Hamburg, Deutschland

2013–2018: Associated Scientist, Diamond Light Source, Harwell, Vereinigtes Königreich

2013–2017: Professor für Strukturelle Zellbiologie, University of Oxford, Oxford, Vereinigtes Königreich

2009–2017: Senior Group Leader und Leiter des Oxford Particle Imaging Centre, University of Oxford, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford, Vereinigtes Königreich

2004–2009: Junior Research Group Leader, Emmy-Noether-Gruppe der Deutschen Forschungsgemeinschaft (DFG), Max-Planck-Institut für Biochemie, Martinsried, Deutschland

2004: Wissenschaftler, Max-Planck-Institut für Biochemie, Martinsried, Deutschland

2002–2003: Postdoktorand, National Institutes of Health, Bethesda, USA, bei Dr. Alasdair C. Steven (Laboratory of Structural Biology Research)

2001: Postdoktorand, Friedrich-Schiller-Universität Jena und Max-Planck-Institut für Biochemie, Martinsried, Deutschland, bei Prof. Wolfgang Baumeister

1997–2000: Promotion in Biologie (Dr. rer. nat.), Friedrich-Schiller-Universität Jena, Deutschland; 1997/1998 Forschungsaufenthalte bei Prof. Joseph Hirschberg, Hebrew University of Jerusalem, Israel

1991–1996: Studium der Biologie (Diplom), Friedrich-Schiller-Universität Jena, Deutschland-2027: Visiting Professor of Structural Cell Biology; University of Oxford, United Kingdom

Since 2014: Faculty Member, Scientific Director of multi-user core facility Electron Cryo-Microscopy (Cryo-EM) and Advanced Light and Fluorescence Microscopy (ALFM) core facility; Centre for Structural Systems Biology (CSSB), Hamburg, Germany

2013-2018: Associated Scientist; Diamond Light Source, Harwell, United Kingdom

2013-2017: Professor of Structural Cell Biology; University of Oxford, United Kingdom

2009-2017: Senior Group Leader and Head of Oxford Particle Imaging Centre; University of Oxford, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, Oxford, United Kingdom

2004-2009: Junior Research Group Leader; Emmy Noether Group of German Research Foundation (DFG), Max Planck Institute of Biochemistry, Martinsried, Germany

2004: Scientist; Max Planck Institute of Biochemistry, Martinsried, Germany

2002-2003: Postdoctoral Fellow; National Institutes of Health, Bethesda, USA

2001: Postdoctoral Fellow; Friedrich Schiller University Jena, and Max Planck Institute of Biochemistry, Martinsried, Germany

1997-2000: Doctorate in Biology (Dr. rer. nat.) with Prof. Wolfram Braune, Friedrich Schiller University Jena, Germany; 1997/1998 research stays, Hebrew University of Jerusalem, Israel

1991-1996: Studies of Biology (Diploma), Friedrich Schiller University Jena, Germany

Publikationen

Corroyer-Dulmont S, Labarde A, Pražák V, Godinho LM, Masson C, Legrand P, Grünewald K, Tavares P, Quemin ERJ (2026). Subcellular reorganization upon phage infection reveals stepwise assembly of viral particles from membrane-associated precursors. Nat Commun. 2026 Apr 02; PubMed

Nentwig J, Grünewald K, Vollmer B (2026). When structural rationales fall short: revisiting the immunogenicity of herpesvirus prefusion and postfusion glycoprotein B. Curr Opin Virol. 76/2026; PubMed

Pražák V, Vasishtan D, Grünewald K, Douglas RG, Ferreira JL (2025). Molecular architecture of glideosome and nuclear F-actin in Plasmodium falciparum. EMBO Rep. 2025 Mar 24; PubMed

Banari A, Samanta AK, Munke A, Laugks T, Bajt S, Grünewald K, Marlovits TC, Küpper J, Maia FRNC, Chapman HN, Oberthür D, Seuring C (2025). Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography. Nat Methods. 2025 May 01; PubMed

Vollmer B, Ebel H, Rees R, Nentwig J, Mulvaney T, Schünemann J, Krull J, Topf M, Görlich D, Grünewald K (2025). A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2. Nature. 2025 Sep 03; PubMed

Wu W, Laugks U, Grünewald K, Roy P (2025). Insect-borne non-enveloped bluetongue virus utilizes discrete small vesicles for non-lytic release and cell-to-cell transmission. PLoS Pathog. 21/2025; PubMed

Baumgartl C, Holzinger S, Schwartz U, Franken LE, Grünewald K, Wodrich H, Längst G (2025). Adenovirus maturation establishes the transcription competent packaging of its genome. EMBO Rep. 2025 Oct 21; PubMed

Gustavsson E, Grünewald K, Elias P, Hällberg BM (2025). The herpes simplex origin-binding protein: mechanisms for sequence-specific DNA binding and dimerization revealed by Cryo-EM. Nucleic Acids Res. 53/2025; PubMed

Manalastas-Cantos K, Adoni KR, Pfeifer M, Märtens B, Grünewald K, Thalassinos K, Topf M (2024). Modeling Flexible Protein Structure With AlphaFold2 and Crosslinking Mass Spectrometry. Mol Cell Proteomics. 23/2024; PubMed

Pražák V, Mironova Y, Vasishtan D, Hagen C, Laugks U, Jensen Y, Sanders S, Heumann JM, Bosse JB, Klupp BG, Mettenleiter TC, Grange M, Grünewald K (2024). Molecular plasticity of herpesvirus nuclear egress analysed in situ. Nat Microbiol. 9/2024; PubMed

Gustavsson E, Grünewald K, Elias P, Hällberg BM (2024). Dynamics of the Herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-reading revealed by Cryo-EM. Nucleic Acids Res. 52/2024; PubMed

Williams HM, Thorkelsson SR, Vogel D, Busch C, Milewski M, Cusack S, Grünewald K, Quemin ERJ, Rosenthal M (2024). Structural snapshots of phenuivirus cap-snatching and transcription. Nucleic Acids Res. 52/2024; PubMed

Liu J, Corroyer-Dulmont S, Pražák V, Khusainov I, Bahrami K, Welsch S, Vasishtan D, Obarska-Kosińska A, Thorkelsson SR, Grünewald K, Quemin ERJ, Turoňová B, Locker JK (2024). The palisade layer of the poxvirus core is composed of flexible A10 trimers. Nat Struct Mol Biol. 31/2024; PubMed

Ott F, Jönsson MR, Grünewald K, Hellert J (2024). Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography. Methods Mol Biol. 2824/2024; PubMed

Hofstadter WA, Cook KC, Tsopurashvili E, Gebauer R, Pražák V, Machala EA, Park JW, Grünewald K, Quemin ERJ, Cristea IM (2024). Infection-induced peripheral mitochondria fission drives ER encapsulations and inter-mitochondria contacts that rescue bioenergetics. Nat Commun. 15/2024; PubMed

Han Y, Hacker D, Donders BC, Parperis C, Thuenauer R, Leterrier C, Grünewald K, Mikhaylova M (2024). Unveiling the cell biology of hippocampal neurons with dendritic axon origin. J Cell Biol. 224/2025; PubMed

Zhou M, Vollmer B, Machala E, Chen M, Grünewald K, Arvin AM, Chiu W, Oliver SL (2023). Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nat Commun. 14/2023; PubMed

Meier K, Thorkelsson SR, Durieux Trouilleton Q, Vogel D, Yu D, Kosinski J, Cusack S, Malet H, Grünewald K, Quemin ERJ, Rosenthal M (2023). Structural and functional characterization of the Sin Nombre virus L protein. PLoS Pathog. 19/2023; PubMed

Ferreira JL, Pražák V, Vasishtan D, Siggel M, Hentzschel F, Binder AM, Pietsch E, Kosinski J, Frischknecht F, Gilberger TW, Grünewald K (2023). Variable microtubule architecture in the malaria parasite. Nat Commun. 14/2023; PubMed

Williams HM, Thorkelsson SR, Vogel D, Milewski M, Busch C, Cusack S, Grünewald K, Quemin ERJ, Rosenthal M (2023). Structural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein. Nucleic Acids Res. 51/2023; PubMed

Franken LE, Rosch R, Laugks U, Grünewald K (2022). Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells. STAR Protoc. 3/2022; PubMed

Bücker R, Seuring C, Cazey C, Veith K, García-Alai M, Grünewald K, Landau M (2022). The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils. Nat Commun. 13/2022; PubMed

Caragliano E, Bonazza S, Frascaroli G, Tang J, Soh TK, Grünewald K, Bosse JB, Brune W (2022). Human cytomegalovirus forms phase-separated compartments at viral genomes to facilitate viral replication. Cell Rep. 38/2022; PubMed

Flomm FJ, Soh TK, Schneider C, Wedemann L, Britt HM, Thalassinos K, Pfitzner S, Reimer R, Grünewald K, Bosse JB (2022). Intermittent bulk release of human cytomegalovirus. PLoS Pathog. 18/2022; PubMed

Villalta A, Schmitt A, Estrozi LF, Quemin ERJ, Alempic JM, Lartigue A, Pražák V, Belmudes L, Vasishtan D, Colmant AMG, Honoré FA, Couté Y, Grünewald K, Abergel C (2022). The giant mimivirus 1.2 Mb genome is elegantly organized into a 30-nm diameter helical protein shield. Elife. 11/2022; PubMed

Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M (2021). Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines. EMBO J. 41/2022; PubMed

Pfitzner S, Bosse JB, Hofmann-Sieber H, Flomm F, Reimer R, Dobner T, Grünewald K, Franken LE (2021). Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. Int J Mol Sci. 22/2021; PubMed

Kouba T, Vogel D, Thorkelsson SR, Quemin ERJ, Williams HM, Milewski M, Busch C, Günther S, Grünewald K, Rosenthal M, Cusack S (2021). Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity. Nat Commun. 12/2021; PubMed

Vallbracht M, Lötzsch H, Klupp BG, Fuchs W, Vollmer B, Grünewald K, Backovic M, Rey FA, Mettenleiter TC (2021). In Vitro Viral Evolution Identifies a Critical Residue in the Alphaherpesvirus Fusion Glycoprotein B Ectodomain That Controls gH/gL-Independent Entry. mBio. 12/2021; PubMed

Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA (2021). DNA origami signposts for identifying proteins on cell membranes by electron cryotomography. Cell. 184/2021; PubMed

Pražák V, Grünewald K, Kaufmann R (2020). Correlative super-resolution fluorescence and electron cryo-microscopy based on cryo-SOFI. Methods Cell Biol. 162/2021; PubMed

Wolff G, Limpens RWAL, Zevenhoven-Dobbe JC, Laugks U, Zheng S, de Jong AWM, Koning RI, Agard DA, Grünewald K, Koster AJ, Snijder EJ, Bárcena M (2020). A molecular pore spans the double membrane of the coronavirus replication organelle. Science. 369/2020; PubMed

Quemin ERJ, Machala EA, Vollmer B, Pražák V, Vasishtan D, Rosch R, Grange M, Franken LE, Baker LA, Grünewald K (2020). Cellular Electron Cryo-Tomography to Study Virus-Host Interactions. Annu Rev Virol. 7/2020; PubMed

Pfitzner S, Hofmann-Sieber H, Bosse JB, Franken LE, Grünewald K, Dobner T (2020). Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'. PLoS Pathog. 16/2020; PubMed

Vogel D, Thorkelsson SR, Quemin ERJ, Meier K, Kouba T, Gogrefe N, Busch C, Reindl S, Günther S, Cusack S, Grünewald K, Rosenthal M (2020). Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein. Nucleic Acids Res. 48/2020; PubMed

Chorev DS, Tang H, Rouse SL, Bolla JR, von Kügelgen A, Baker LA, Wu D, Gault J, Grünewald K, Bharat TAM, Matthews SJ, Robinson CV (2020). The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes. Nat Protoc. 15/2020; PubMed

Franken LE, Grünewald K, Boekema EJ, Stuart MCA (2020). A Technical Introduction to Transmission Electron Microscopy for Soft-Matter: Imaging, Possibilities, Choices, and Technical Developments. Small. 16/2020; PubMed

Vollmer B, Grünewald K (2020). Herpesvirus membrane fusion - a team effort. Curr Opin Struct Biol. 62/2020; PubMed

Vollmer B, Pražák V, Vasishtan D, Jefferys EE, Hernandez-Duran A, Vallbracht M, Klupp BG, Mettenleiter TC, Backovic M, Rey FA, Topf M, Grünewald K (2020). The prefusion structure of herpes simplex virus glycoprotein B. Sci Adv. 6/2020; PubMed

Hernández Durán A, Grünewald K, Topf M (2019). Conserved Central Intraviral Protein Interactome of the Herpesviridae Family. mSystems. 4/2019; PubMed

Hernández Durán A, Greco TM, Vollmer B, Cristea IM, Grünewald K, Topf M (2019). Protein interactions and consensus clustering analysis uncover insights into herpesvirus virion structure and function relationships. PLoS Biol. 17/2019; PubMed

Moser F, Pražák V, Mordhorst V, Andrade DM, Baker LA, Hagen C, Grünewald K, Kaufmann R (2019). Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy. Proc Natl Acad Sci U S A. 116/2019; PubMed

Grünewald K, Krey T (2018). Editorial Overview. Curr Opin Virol. 31/2018; PubMed

Chorev DS, Baker LA, Wu D, Beilsten-Edmands V, Rouse SL, Zeev-Ben-Mordehai T, Jiko C, Samsudin F, Gerle C, Khalid S, Stewart AG, Matthews SJ, Grünewald K, Robinson CV (2018). Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry. Science. 362/2018; PubMed

Ando T, Bhamidimarri SP, Brending N, Colin-York H, Collinson L, De Jonge N, de Pablo PJ, Debroye E, Eggeling C, Franck C, Fritzsche M, Gerritsen H, Giepmans BNG, Grunewald K, Hofkens J, Hoogenboom JP, Janssen KPF, Kaufman R, Klumpermann J, Kurniawan N, Kusch J, Liv N, Parekh V, Peckys DB, Rehfeldt F, Reutens DC, Roeffaers MBJ, Salditt T, Schaap IAT, Schwarz US, Verkade P, Vogel MW, Wagner R, Winterhalter M, Yuan H, Zifarelli G (2018). The 2018 correlative microscopy techniques roadmap. J Phys D Appl Phys. 51/2018; PubMed

Montespan C, Marvin SA, Austin S, Burrage AM, Roger B, Rayne F, Faure M, Campell EM, Schneider C, Reimer R, Grünewald K, Wiethoff CM, Wodrich H (2017). Multi-layered control of Galectin-8 mediated autophagy during adenovirus cell entry through a conserved PPxY motif in the viral capsid. PLoS Pathog. 13/2017; PubMed